Photolithographic Fabrication of Micro Apertures in Dry Film Polymer Sheets for Channel Recordings in Planar Lipid Bilayers
نویسندگان
چکیده
منابع مشابه
Microfabricated teflon membranes for low-noise recordings of ion channels in planar lipid bilayers.
We present a straightforward, accessible method for the fabrication of micropores with diameters from 2 to 800 micro m in films of amorphous Teflon (Teflon AF). Pores with diameters </=40 micro m made it possible to record ion fluxes through ion channels in planar bilayers with excellent signal characteristics. These pores afforded: i), stable measurements at transmembrane voltages up to 460 mV...
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ژورنال
عنوان ژورنال: The Journal of Membrane Biology
سال: 2019
ISSN: 0022-2631,1432-1424
DOI: 10.1007/s00232-019-00062-9